Dataset Information
Single-Particle CryoEM of mammalian V-ATPase with the TLDc domain protein mEAK7 bound (Various Datasets)
Ontology highlight
ABSTRACT:
SUBMITTER: Yong Zi Tan
PROVIDER: EMPIAR-10874 | biostudies-other |
REPOSITORIES: biostudies-other
Publications
CryoEM of endogenous mammalian V-ATPase interacting with the TLDc protein mEAK-7.
Life science alliance 20220706 11
V-ATPases are rotary proton pumps that serve as signaling hubs with numerous protein binding partners. CryoEM with exhaustive focused classification allowed detection of endogenous proteins associated with porcine kidney V-ATPase. An extra C subunit was found in ∼3% of complexes, whereas ∼1.6% of complexes bound mEAK-7, a protein with proposed roles in dauer formation in nematodes and mTOR signaling in mammals. High-resolution cryoEM of porcine kidney V-ATPase with recombinant mEAK-7 showed that ...[more]