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Single-Particle CryoEM of mammalian V-ATPase with the TLDc domain protein mEAK7 bound (Various Datasets)

ABSTRACT:

SUBMITTER: Yong Zi Tan

PROVIDER: EMPIAR-10874 | biostudies-other |

REPOSITORIES: biostudies-other

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CryoEM of endogenous mammalian V-ATPase interacting with the TLDc protein mEAK-7.

Tan Yong Zi YZ Abbas Yazan M YM Wu Jing Ze JZ Wu Di D Keon Kristine A KA Hesketh Geoffrey G GG Bueler Stephanie A SA Gingras Anne-Claude AC Robinson Carol V CV Grinstein Sergio S Rubinstein John L JL

Life science alliance 20220706 11

V-ATPases are rotary proton pumps that serve as signaling hubs with numerous protein binding partners. CryoEM with exhaustive focused classification allowed detection of endogenous proteins associated with porcine kidney V-ATPase. An extra C subunit was found in ∼3% of complexes, whereas ∼1.6% of complexes bound mEAK-7, a protein with proposed roles in dauer formation in nematodes and mTOR signaling in mammals. High-resolution cryoEM of porcine kidney V-ATPase with recombinant mEAK-7 showed that ...[more]

PMID: 35794005

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