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Structure of transcription factor UAF in complex with TBP and 35S rRNA promoter DNA

ABSTRACT:

SUBMITTER: Christoph W Müller

PROVIDER: EMPIAR-10964 | biostudies-other |

REPOSITORIES: biostudies-other

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Mechanism of RNA polymerase I selection by transcription factor UAF.

Baudin Florence F Murciano Brice B Fung Herman K H HKH Fromm Simon A SA Mattei Simone S Mahamid Julia J Müller Christoph W CW

Science advances 20220420 16

Preribosomal RNA is selectively transcribed by RNA polymerase (Pol) I in eukaryotes. The yeast transcription factor upstream activating factor (UAF) represses Pol II transcription and mediates Pol I preinitiation complex (PIC) formation at the 35<i>S</i> ribosomal RNA gene. To visualize the molecular intermediates toward PIC formation, we determined the structure of UAF in complex with native promoter DNA and transcription factor TATA-box-binding protein (TBP). We found that UAF recognizes DNA u ...[more]

PMID: 35442737

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