Infectious mouse-adapted RML scrapie prion fibrils purified from terminally-infected mouse brains
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ABSTRACT:
SUBMITTER: Szymon W Manka
PROVIDER: EMPIAR-10992 | biostudies-other |
REPOSITORIES: biostudies-other
Publications
2.7 Å cryo-EM structure of ex vivo RML prion fibrils.
Manka Szymon W SW Zhang Wenjuan W Wenborn Adam A Betts Jemma J Joiner Susan S Saibil Helen R HR Collinge John J Wadsworth Jonathan D F JDF
Nature communications 20220713 1
Mammalian prions propagate as distinct strains and are composed of multichain assemblies of misfolded host-encoded prion protein (PrP). Here, we present a near-atomic resolution cryo-EM structure of PrP fibrils present in highly infectious prion rod preparations isolated from the brains of RML prion-infected mice. We found that prion rods comprise single-protofilament helical amyloid fibrils that coexist with twisted pairs of the same protofilaments. Each rung of the protofilament is formed by a ...[more]
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