Dataset Information
Single particle cryo-EM dataset of cowpea chlorotic mottle virus in laser-melted and revitrified ice
Ontology highlight
ABSTRACT:
SUBMITTER: Ulrich J Lorenz
PROVIDER: EMPIAR-11019 | biostudies-other |
REPOSITORIES: biostudies-other
Publications
Microsecond melting and revitrification of cryo samples: protein structure and beam-induced motion.
Acta crystallographica. Section D, Structural biology 20220614 Pt 7
A novel approach to time-resolved cryo-electron microscopy (cryo-EM) has recently been introduced that involves melting a cryo sample with a laser beam to allow protein dynamics to briefly occur in the liquid, before trapping the particles in their transient configurations by rapidly revitrifying the sample. With a time resolution of just a few microseconds, this approach is notably fast enough to study the domain motions that are typically associated with the activity of proteins but which have ...[more]