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Cryo-electron microscopy of Dicer-1 and Its Partner Protein Loqs-PB complex with model pre-miRNA in presence of Mg2+ ions

ABSTRACT:

SUBMITTER: Andrei Korostelev

PROVIDER: EMPIAR-11234 | biostudies-other |

REPOSITORIES: biostudies-other

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Structural basis of microRNA biogenesis by Dicer-1 and its partner protein Loqs-PB.

Jouravleva Karina K Golovenko Dmitrij D Demo Gabriel G Dutcher Robert C RC Hall Traci M Tanaka TMT Zamore Phillip D PD Korostelev Andrei A AA

Molecular cell 20220930 21

In animals and plants, Dicer enzymes collaborate with double-stranded RNA-binding domain (dsRBD) proteins to convert precursor-microRNAs (pre-miRNAs) into miRNA duplexes. We report six cryo-EM structures of Drosophila Dicer-1 that show how Dicer-1 and its partner Loqs‑PB cooperate (1) before binding pre-miRNA, (2) after binding and in a catalytically competent state, (3) after nicking one arm of the pre-miRNA, and (4) following complete dicing and initial product release. Our reconstructions sug ...[more]

PMID: 36182693

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