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Quantification of gallium cryo-FIB milling damage in biological lamella

ABSTRACT:

SUBMITTER: Bronwyn Ayla Lucas

PROVIDER: EMPIAR-11544 | biostudies-other |

REPOSITORIES: biostudies-other

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Quantification of gallium cryo-FIB milling damage in biological lamellae.

Lucas Bronwyn A BA Grigorieff Nikolaus N

Proceedings of the National Academy of Sciences of the United States of America 20230522 23

Cryogenic electron microscopy (cryo-EM) can reveal the molecular details of biological processes in their native, cellular environment at atomic resolution. However, few cells are sufficiently thin to permit imaging with cryo-EM. Thinning of frozen cells to <500 nm lamellae by focused-ion-beam (FIB) milling has enabled visualization of cellular structures with cryo-EM. FIB milling represents a significant advance over prior approaches because of its ease of use, scalability, and lack of large-sc ...[more]

PMID: 37216561

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