Dataset Information
Quantification of gallium cryo-FIB milling damage in biological lamella
Ontology highlight
ABSTRACT:
SUBMITTER: Bronwyn Ayla Lucas
PROVIDER: EMPIAR-11544 | biostudies-other |
REPOSITORIES: biostudies-other
Publications
Quantification of gallium cryo-FIB milling damage in biological lamellae.
Proceedings of the National Academy of Sciences of the United States of America 20230522 23
Cryogenic electron microscopy (cryo-EM) can reveal the molecular details of biological processes in their native, cellular environment at atomic resolution. However, few cells are sufficiently thin to permit imaging with cryo-EM. Thinning of frozen cells to <500 nm lamellae by focused-ion-beam (FIB) milling has enabled visualization of cellular structures with cryo-EM. FIB milling represents a significant advance over prior approaches because of its ease of use, scalability, and lack of large-sc ...[more]