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Cryo-EM micrographs of Tb ADAT2/3 bound to tRNA

ABSTRACT:

SUBMITTER: Luciano Graciani Dolce

PROVIDER: EMPIAR-11673 | biostudies-other |

REPOSITORIES: biostudies-other

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Structural basis for sequence-independent substrate selection by eukaryotic wobble base tRNA deaminase ADAT2/3.

Dolce Luciano G LG Zimmer Aubree A AA Tengo Laura L Weis Félix F Rubio Mary Anne T MAT Alfonzo Juan D JD Kowalinski Eva E

Nature communications 20221108 1

The essential deamination of adenosine A<sub>34</sub> to inosine at the wobble base is the individual tRNA modification with the greatest effects on mRNA decoding, empowering a single tRNA to translate three different codons. To date, many aspects of how eukaryotic deaminases specifically select their multiple substrates remain unclear. Here, using cryo-EM, we present the structure of a eukaryotic ADAT2/3 deaminase bound to a full-length tRNA, revealing that the enzyme distorts the anticodon loo ...[more]

PMID: 36347890

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