Dataset Information
Single-Particle Cryo-EM of RNA Polymerase at Various Tilts
Ontology highlight
ABSTRACT:
SUBMITTER: Yong Zi Tan
PROVIDER: EMPIAR-11797 | biostudies-other |
REPOSITORIES: biostudies-other
Publications
Overcoming resolution attenuation during tilted cryo-EM data collection.
Nature communications 20240109 1
Structural biology efforts using cryogenic electron microscopy are frequently stifled by specimens adopting "preferred orientations" on grids, leading to anisotropic map resolution and impeding structure determination. Tilting the specimen stage during data collection is a generalizable solution but has historically led to substantial resolution attenuation. Here, we develop updated data collection and image processing workflows and demonstrate, using multiple specimens, that resolution attenuat ...[more]