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Tomograms and the raw 2d tilt series of ER-ER junctions of HeLa cells in interphase

ABSTRACT:

SUBMITTER: Shotaro Otsuka

PROVIDER: EMPIAR-12050 | biostudies-other |

REPOSITORIES: biostudies-other

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The endoplasmic reticulum connects to the nucleus by constricted junctions that mature after mitosis.

Bragulat-Teixidor Helena H Ishihara Keisuke K Szücs Gréta Martina GM Otsuka Shotaro S

EMBO reports 20240614 7

Junctions between the endoplasmic reticulum (ER) and the outer membrane of the nuclear envelope (NE) physically connect both organelles. These ER-NE junctions are essential for supplying the NE with lipids and proteins synthesized in the ER. However, little is known about the structure of these ER-NE junctions. Here, we systematically study the ultrastructure of ER-NE junctions in cryo-fixed mammalian cells staged in anaphase, telophase, and interphase by correlating live cell imaging with three ...[more]

PMID: 38877171

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