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Raw 2d tomographic tilt series of a dividing cell

ABSTRACT:

SUBMITTER: Jan Ellenberg

PROVIDER: EMPIAR-10116 | biostudies-other |

REPOSITORIES: biostudies-other

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Postmitotic nuclear pore assembly proceeds by radial dilation of small membrane openings.

Otsuka Shotaro S Steyer Anna M AM Schorb Martin M Hériché Jean-Karim JK Hossain M Julius MJ Sethi Suruchi S Kueblbeck Moritz M Schwab Yannick Y Beck Martin M Ellenberg Jan J

Nature structural & molecular biology 20171127 1

The nuclear envelope has to be reformed after mitosis to create viable daughter cells with closed nuclei. How membrane sealing of DNA and assembly of nuclear pore complexes (NPCs) are achieved and coordinated is poorly understood. Here, we reconstructed nuclear membrane topology and the structures of assembling NPCs in a correlative 3D EM time course of dividing human cells. Our quantitative ultrastructural analysis shows that nuclear membranes form from highly fenestrated ER sheets whose holes ...[more]

PMID: 29323269

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Automated tilt series alignment and tomographic reconstruction in IMOD.

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| S-EPMC5247408 | biostudies-literature

Determination of Antibody Conformation from Tomographic Tilt Series