Cryo-EM structure of guide RNA and target RNA bound Cas7-11
Ontology highlight
ABSTRACT:
SUBMITTER: Hong Li
PROVIDER: EMPIAR-12113 | biostudies-other |
REPOSITORIES: biostudies-other
Publications
Molecular mechanism of active Cas7-11 in processing CRISPR RNA and interfering target RNA.
Goswami Hemant N HN Rai Jay J Das Anuska A Li Hong H
eLife 20221003
Cas7-11 is a Type III-E CRISPR Cas effector that confers programmable RNA cleavage and has potential applications in RNA interference. Cas7-11 encodes a single polypeptide containing four Cas7- and one Cas11-like segments that obscures the distinction between the multi-subunit Class 1 and the single-subunit Class-2 CRISPR Cas systems. We report a cryo-EM (cryo-electron microscopy) structure of the active Cas7-11 from <i>Desulfonema ishimotonii</i> (DiCas7-11) that reveals the molecular basis for ...[more]
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