Dataset Information
CryoEM of E. coli RNA polymerase on T7A1 promoter DNA
Ontology highlight
ABSTRACT:
SUBMITTER: Seth A Darst
PROVIDER: EMPIAR-12319 | biostudies-other |
REPOSITORIES: biostudies-other
Publications
Structural origins of <i>Escherichia coli</i> RNA polymerase open promoter complex stability.
Proceedings of the National Academy of Sciences of the United States of America 20211001 40
The first step in gene expression in all organisms requires opening the DNA duplex to expose one strand for templated RNA synthesis. In <i>Escherichia coli</i>, promoter DNA sequence fundamentally determines how fast the RNA polymerase (RNAP) forms "open" complexes (RPo), whether RPo persists for seconds or hours, and how quickly RNAP transitions from initiation to elongation. These rates control promoter strength in vivo, but their structural origins remain largely unknown. Here, we use cryoele ...[more]