Dataset Information
CryoEM of E. coli RNA polymerase and lambda PR promoter DNA at equilibrium at 5 degrees Celsius
Ontology highlight
ABSTRACT:
SUBMITTER: Seth A Darst
PROVIDER: EMPIAR-12338 | biostudies-other |
REPOSITORIES: biostudies-other
Publications
Early intermediates in bacterial RNA polymerase promoter melting visualized by time-resolved cryo-electron microscopy.
Nature structural & molecular biology 20240701 11
During formation of the transcription-competent open complex (RPo) by bacterial RNA polymerases (RNAPs), transient intermediates pile up before overcoming a rate-limiting step. Structural descriptions of these interconversions in real time are unavailable. To address this gap, here we use time-resolved cryogenic electron microscopy (cryo-EM) to capture four intermediates populated 120 ms or 500 ms after mixing Escherichia coli σ<sup>70</sup>-RNAP and the λP<sub>R</sub> promoter. Cryo-EM snapshot ...[more]