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CryoEM of E. coli RNA polymerase on lambda PR promoter DNA


ABSTRACT:

SUBMITTER: Seth A Darst 

PROVIDER: EMPIAR-12324 | biostudies-other |

REPOSITORIES: biostudies-other

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Publications

Structural origins of <i>Escherichia coli</i> RNA polymerase open promoter complex stability.

Saecker Ruth M RM   Chen James J   Chiu Courtney E CE   Malone Brandon B   Sotiris Johanna J   Ebrahim Mark M   Yen Laura Y LY   Eng Edward T ET   Darst Seth A SA  

Proceedings of the National Academy of Sciences of the United States of America 20211001 40


The first step in gene expression in all organisms requires opening the DNA duplex to expose one strand for templated RNA synthesis. In <i>Escherichia coli</i>, promoter DNA sequence fundamentally determines how fast the RNA polymerase (RNAP) forms "open" complexes (RPo), whether RPo persists for seconds or hours, and how quickly RNAP transitions from initiation to elongation. These rates control promoter strength in vivo, but their structural origins remain largely unknown. Here, we use cryoele  ...[more]

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