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Figure 4A HA@PPCDQ induces CD4+ and CD8+ effector memory T cells (TEM, CD62L-CD44+) generation at 2 weeks after boost vaccination.

ABSTRACT: C57BL/6 mice (n = 6) were immunized with PPCDQ nanovaccines or soluble protein, respectively. Splenocytes were isolated at 14 days post-boost and stained with cocktails of T cell surface markers for FCM analysis.

ORGANISM(S): Mus musculus (mouse)

SUBMITTER:

PROVIDER: S-BSST1386 | biostudies-other |

REPOSITORIES: biostudies-other

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Figure 4C HA@PPCDQ induces CD4+ effector memory T cells (TCM, CD62L+CD44+) generation at 2 weeks after boost vaccination.

Project description: Not available

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Figure 4D. HA@PPCDQ induces CD8+ effector memory T cells (TCM, CD62L+CD44+) generation at 2 weeks after boost vaccination.

Project description: Not available

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Figure 5G. HA@PPCDQ induces higher frequency of HA-specific MBC at 2 weeks after boost vaccination.

Project description: Not available

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Figure 5D. HA@PPCDQ induces higher frequency of HA-specific GC Tfh (CD25+OX40+) cells at 1 weeks after boost vaccination.

Project description: Not available

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Figure 2A HA@PPCDQ facilitates antigen uptake in vivo.

Project description: Not available

| S-BSST1385 | biostudies-other

Transcriptomic evaluation of CD4+CD62L-CD44+FoxP3-CD45RbLo (RbLo TEM) versus CD4+CD62L+CD44-FoxP-CD45RbHi (RbHi TN) cells following activation

Project description:Deep sequencing of splenic RbLo TEM and RbHi TN cells 72 hours following anti-CD3 stimulation.

2019-06-10 | GSE89241 | GEO

Figure 3E and 3F. HA@PPCDQ immunization promotes neutrophil recruitment and depletion of CD169+ macrophages in spleen.

Project description: Not available

| S-BSST1384 | biostudies-other

Comparison of naÃ¯ve, postmitotic (CD44-lo/CD62L-hi) and effector (CD44-hi/CD62L-lo) CD8+ Tcells during BMT in GVHD mice

Project description:NaÃ¯ve CD44-lo/CD62L-hi/CD8+ T cells from C3H.SW mice were compared to CD44-hi/CD82L-lo/CD8+ effector memory T cells and CD44-lo/CD62L-hi/CD8+ postmitotic T cells, using 3 biological replicates of each type of sample. The later two cells types were highly purified at day 14 after transplantation from GVHD B6/SJL mice receiving donor C3H.SW mouse-derived naive CD44-lo/CD62L-hi/CD8+ T cells and T cell-depleted bone marrow. Recipient mice had first been lethally irradiated at a dose of 10Gy in two fractions. This is a MHC-identical minor histocompatibility antigen-mismatched mouse GVHD model of human allogeneic hematopoietic stem cell transplantation. Naive T cell samples were from pools of 2 mice each, while effector memory and postmitotic T cell samples were purified from pools of T cells from 4 mice each. After RNA extraction and cleanup, biotin labeled cRNA was prepared from 600 ng total RNA, using two rounds of in vitro transcription, and hybridized to Affymetrix Mouse Genome 430A 2.0 arrays using standard techniques. Keywords: Cell type comparison 9 samples were analyzed on 9 Affymetrix microarrays to assay mRNA levels. There were 3 biological replicates of each of 3 different cell types.

2010-06-08 | E-GEOD-13743 | biostudies-arrayexpress

Comparison of naïve, postmitotic (CD44-lo/CD62L-hi) and effector (CD44-hi/CD62L-lo) CD8+ Tcells during BMT in GVHD mice

Project description:Naïve CD44-lo/CD62L-hi/CD8+ T cells from C3H.SW mice were compared to CD44-hi/CD82L-lo/CD8+ effector memory T cells and CD44-lo/CD62L-hi/CD8+ postmitotic T cells, using 3 biological replicates of each type of sample. The later two cells types were highly purified at day 14 after transplantation from GVHD B6/SJL mice receiving donor C3H.SW mouse-derived naive CD44-lo/CD62L-hi/CD8+ T cells and T cell-depleted bone marrow. Recipient mice had first been lethally irradiated at a dose of 10Gy in two fractions. This is a MHC-identical minor histocompatibility antigen-mismatched mouse GVHD model of human allogeneic hematopoietic stem cell transplantation. Naive T cell samples were from pools of 2 mice each, while effector memory and postmitotic T cell samples were purified from pools of T cells from 4 mice each. After RNA extraction and cleanup, biotin labeled cRNA was prepared from 600 ng total RNA, using two rounds of in vitro transcription, and hybridized to Affymetrix Mouse Genome 430A 2.0 arrays using standard techniques. Keywords: Cell type comparison

2010-05-21 | GSE13743 | GEO

CD44/HA signaling mediates acquired resistance to a PI3Kα inhibitor.

Project description:Most luminal breast carcinomas (BrCas) bearing PIK3CA mutations initially respond to phosphoinositide-3-kinase (PI3K)-α inhibitors, but many eventually become resistant. The underlying mechanisms of this resistance remain obscure. In this work, we showed that a CD44high state due to aberrant isoform splicing was acquired from adaptive resistance to a PI3Kα inhibitor (BLY719) in luminal BrCas. Notably, the expression of CD44 was positively correlated with estrogen receptor (ER) activity in PIK3CA-mutant breast cancers, and ER-dependent transcription upon PI3Kα pathway inhibition was in turn mediated by CD44. Furthermore, the interaction of CD44 with the ligand hyaluronan (HA) initiated the Src-ERK signaling cascade, which subsequently maintained AKT and mTOR activity in the presence of a PI3Kα inhibitor. Activation of this pathway was prevented by disruption of the CD44/HA interaction, which in turn restored sensitivity to BLY719. Our results revealed that an ER-CD44-HA signaling circuit that mediates robust compensatory activation of the Src-ERK signaling cascade may contribute to the development of acquired resistance to PI3Kα inhibitors. This study provides new insight into the mechanism of adaptive resistance to PI3Kα inhibition therapy.

| S-EPMC7538592 | biostudies-literature

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