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Figure 4D. HA@PPCDQ induces CD8+ effector memory T cells (TCM, CD62L+CD44+) generation at 2 weeks after boost vaccination.

ABSTRACT: C57BL/6 mice (n = 6) were immunized with PPCDQ nanovaccines or soluble protein, respectively. Splenocytes were isolated at 14 days post-boost and stained with cocktails of T cell surface markers for FCM analysis

ORGANISM(S): Mus musculus (mouse)

SUBMITTER:

PROVIDER: S-BSST1388 | biostudies-other |

REPOSITORIES: biostudies-other

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Figure 4C HA@PPCDQ induces CD4+ effector memory T cells (TCM, CD62L+CD44+) generation at 2 weeks after boost vaccination.

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Figure 4A HA@PPCDQ induces CD4+ and CD8+ effector memory T cells (TEM, CD62L-CD44+) generation at 2 weeks after boost vaccination.

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Figure 5G. HA@PPCDQ induces higher frequency of HA-specific MBC at 2 weeks after boost vaccination.

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Figure 5D. HA@PPCDQ induces higher frequency of HA-specific GC Tfh (CD25+OX40+) cells at 1 weeks after boost vaccination.

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Figure 2A HA@PPCDQ facilitates antigen uptake in vivo.

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Comparison of naÃ¯ve, postmitotic (CD44-lo/CD62L-hi) and effector (CD44-hi/CD62L-lo) CD8+ Tcells during BMT in GVHD mice

Project description:NaÃ¯ve CD44-lo/CD62L-hi/CD8+ T cells from C3H.SW mice were compared to CD44-hi/CD82L-lo/CD8+ effector memory T cells and CD44-lo/CD62L-hi/CD8+ postmitotic T cells, using 3 biological replicates of each type of sample. The later two cells types were highly purified at day 14 after transplantation from GVHD B6/SJL mice receiving donor C3H.SW mouse-derived naive CD44-lo/CD62L-hi/CD8+ T cells and T cell-depleted bone marrow. Recipient mice had first been lethally irradiated at a dose of 10Gy in two fractions. This is a MHC-identical minor histocompatibility antigen-mismatched mouse GVHD model of human allogeneic hematopoietic stem cell transplantation. Naive T cell samples were from pools of 2 mice each, while effector memory and postmitotic T cell samples were purified from pools of T cells from 4 mice each. After RNA extraction and cleanup, biotin labeled cRNA was prepared from 600 ng total RNA, using two rounds of in vitro transcription, and hybridized to Affymetrix Mouse Genome 430A 2.0 arrays using standard techniques. Keywords: Cell type comparison 9 samples were analyzed on 9 Affymetrix microarrays to assay mRNA levels. There were 3 biological replicates of each of 3 different cell types.

2010-06-08 | E-GEOD-13743 | biostudies-arrayexpress

Figure 3E and 3F. HA@PPCDQ immunization promotes neutrophil recruitment and depletion of CD169+ macrophages in spleen.

Project description: Not available

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Comparison of naïve, postmitotic (CD44-lo/CD62L-hi) and effector (CD44-hi/CD62L-lo) CD8+ Tcells during BMT in GVHD mice

Project description:Naïve CD44-lo/CD62L-hi/CD8+ T cells from C3H.SW mice were compared to CD44-hi/CD82L-lo/CD8+ effector memory T cells and CD44-lo/CD62L-hi/CD8+ postmitotic T cells, using 3 biological replicates of each type of sample. The later two cells types were highly purified at day 14 after transplantation from GVHD B6/SJL mice receiving donor C3H.SW mouse-derived naive CD44-lo/CD62L-hi/CD8+ T cells and T cell-depleted bone marrow. Recipient mice had first been lethally irradiated at a dose of 10Gy in two fractions. This is a MHC-identical minor histocompatibility antigen-mismatched mouse GVHD model of human allogeneic hematopoietic stem cell transplantation. Naive T cell samples were from pools of 2 mice each, while effector memory and postmitotic T cell samples were purified from pools of T cells from 4 mice each. After RNA extraction and cleanup, biotin labeled cRNA was prepared from 600 ng total RNA, using two rounds of in vitro transcription, and hybridized to Affymetrix Mouse Genome 430A 2.0 arrays using standard techniques. Keywords: Cell type comparison

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Transcriptomic evaluation of CD4+CD62L-CD44+FoxP3-CD45RbLo (RbLo TEM) versus CD4+CD62L+CD44-FoxP-CD45RbHi (RbHi TN) cells following activation

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