Unknown

Dataset Information

0

Interaction of bleomycin with a bent DNA fragment.


ABSTRACT: The interaction of bleomycin with a kinetoplast DNA fragment has been examined using various footprinting techniques. This DNA adopts a bent structure and displays an unusually low gel mobility on account of its phased runs of adenines. The bleomycin-cobalt complex increases the mobility of this DNA fragment, in contrast with other DNAs which show a decreased rate of gel migration, suggesting that the antibiotic removes DNA bending, possibly via an unwinding mechanism. Removal of the bending is confirmed by hydroxy-radical footprinting which produces a more even ladder of bands in the presence of the ligand. Cleavage by bleomycin is at the sequence G-pyrimidine, though not all such sites are affected to the same extent and some cutting is found at GA and GG. DNase I footprinting confirms the antibiotic-binding sites but reveals that some strong cleavage sites do not yield footprints. Bleomycin renders adenines on the 3' side of its cleavage sites (GT, GC and GA) hyper-reactive to diethyl pyrocarbonate.

SUBMITTER: Nightingale KP 

PROVIDER: S-EPMC1132629 | biostudies-other | 1992 Jun

REPOSITORIES: biostudies-other

Similar Datasets

| S-EPMC1131555 | biostudies-other
| S-EPMC6961295 | biostudies-literature
| S-EPMC3840713 | biostudies-literature
| S-EPMC9293343 | biostudies-literature
| S-EPMC7323118 | biostudies-literature
| S-EPMC4675846 | biostudies-literature
| S-EPMC7309149 | biostudies-literature
| S-EPMC1069516 | biostudies-literature
| S-EPMC4179586 | biostudies-literature
| S-EPMC3422780 | biostudies-literature