Glucose- and insulin-independent induction of ATP citrate lyase in primary cultures of rat hepatocytes.
Ontology highlight
ABSTRACT:
SUBMITTER: Katz NR
PROVIDER: S-EPMC1152010 | biostudies-other | 1983 Apr
REPOSITORIES: biostudies-other
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Project description:Human ATP-citrate lyase (EC 2.3.3.8) is the cytoplasmic enzyme that catalyzes the production of acetyl-CoA from citrate, CoA and ATP. The amino-terminal portion of the enzyme, containing residues 1-817, was crystallized in the presence of tartrate, ATP and magnesium ions. The crystals diffracted to 2.3 Å resolution. The structure shows ADP-Mg(2+) bound to the domain that possesses the ATP-grasp fold. The structure demonstrates that this crystal form could be used to investigate the structures of complexes with inhibitors of ATP-citrate lyase that bind at either the citrate- or ATP-binding site.
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