Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Evidence against protein-induced 'internal pressure' in biological membranes. Partition of 8-anilinonaphthalene-1-sulphonate into Triton X-100 micelles and submitochondrial particles.

ABSTRACT: It has recently been proposed that although small amphiphilic molecules partition into phospholipid vesicles this partition is reduced by a factor of 10(3)-10(4)-fold by the presence of proteins in biological membranes [Conrad & Singer (1979) Proc. Natl. Acad. Sci. U.S.A.76, 5202-5206 and (1981) Biochemistry20, 808-818]. However, the affinity with which 8-anilinonaphthalene-1-sulphonate partitions into, or binds to, Triton X-100 micelles and submitochondrial particles is very similar and therefore does not support this proposal.

SUBMITTER: Gains N

PROVIDER: S-EPMC1153899 | biostudies-other | 1982 Dec

REPOSITORIES: biostudies-other

ACCESS DATA

Json Xml

Similar Datasets

A kinetic analysis of the changes in fluorescence on the interaction of 8-anilinonaphthalene-1-sulphonate with submitochondrial particles.

Project description:A comparison of the fluorescence change on the addition of 8-anilinonaphthalene-1-sulphonate to succinate-energized submitochondrial particles with that on the addition of succinate to submitochondrial particles incubated with 8-anilinonaphthalene-1-sulphonate shows that these changes in fluorescence may be explained solely in terms of 8-anilinonaphthalene-1-sulphonate binding. This comparison does not support the proposal of an 8-anilinonaphthalene-1-sulphonate-monitored change in the conformation of submitochondrial-particle membranes [Brocklehurst, Freedman, Hancock & Radda (1970) Biochem. J.116, 721-731]. The biphasic nature of the decrease in fluorescence, which was found to follow the addition of uncoupler to submitochondrial particles incubated with ATP or succinate, or of antimycin A to submitochondrial particles incubated with succinate, does not support the existence of 'aplectic' and 'symplectic' states of the mitochondrial membrane [Barrett-Bee & Radda (1972) Biochim, Biophys. Acta 267, 211-215].

| S-EPMC1163971 | biostudies-other

Composition of Surface Layer at the Water-Air Interface and Micelles of Triton X-100 + Rhamnolipid Mixtures.

Project description:Measurements of the surface tensions, densities and viscosities of aqueous solutions of Triton X-100 (TX-100) and rhamnolipid (RL) mixtures, at constant concentration of RL or TX-100, were carried out. The measured values of the surface tension were compared to those determined using different theoretical models and on the basis of the surface tension of aqueous solutions of individual surfactants. From the surface tension isotherms, the Gibbs surface excess concentration of TX-100 and RL, the composition of surface layer and the standard Gibbs free energy of adsorption at the water-air interface were determined. Moreover, on the basis of surface tension, density and viscosity isotherms, the CMC of surfactants mixtures were evaluated. From the density isotherms, apparent and partial molar volumes of TX-100 and RL were also determined. These volumes were compared to those calculated from the sizes of TX-100 and RL molecules. There was observed a synergetic effect in the reduction of water surface tension and micelle formation, which was confirmed by the intermolecular interactions parameter. In the case of micelle formation, this effect was discussed based on the standard Gibbs free energy of micellization as well as of TX-100 and RL mixing in the micelles. The synergism of TX-100 and RL mixtures in the reduction of water surface tension and micelle formation was explained on the basis of electrostatic interactions between the hydrophilic part of TX-100 and RL molecules; this was supported by pH measurements.

| S-EPMC5488207 | biostudies-literature

The Effect of Residual Triton X-100 on Structural Stability and Infection Activity of Adenovirus Particles.

Project description:To ensure the high purity and biological activity of the adenovirus vector to be used for clinical applications, a stable and linearly scalable preparation method is highly imperative. During the adenovirus-harvesting process, the Triton X-100-based lysis method possesses the advantages of higher efficiency as well as easier linearization and amplification. Most Triton X-100 can be removed from the adenovirus sample by chromatographic purification. However, there is no report that a small amount of residual Triton X-100, present in adenovirus sample, can affect the particle integrity, infectivity, and structure of adenoviruses. Here, we found that although residual Triton X-100 affected the short-term stability, purity, infectivity, and structure of adenoviruses at 37°C, it did not hamper these properties of adenoviruses at 4°C. This study suggests that although the Triton X-100-based lysis method is a simple, efficient, and easy-to-scale process for lysing host cells to release the adenovirus, the storage conditions of adenovirus products must be taken into consideration.

| S-EPMC7490641 | biostudies-literature

Electron paramagnetic resonance study of the surface hydration of Triton X-100 micelles in water with added monovalent alkali salts.

Project description:The hydrophobic spin probe 2,2,6,6-tetramethyl-piperidin-1-oxyl-4-yl octadecanoate (TEMPO-stearate) was used to study the hydration of the polar shell of Triton X-100 micelles as functions of the concentration of the electrolytes KCl, NaCl, and LiCl and temperature. It was shown that the hydration of the polar shell of the Triton X-100 micelle decreases with both increasing electrolyte concentration and increasing temperature. The effect of Li(+) on the hydration of the polar shell was found to be smaller than those of Na(+) and K(+), which have almost identical behavior. The effective water concentration decreases from 18.3 to 15.8 M for LiCl and from 18.3 to 13.9 M for NaCl and KCl when the concentration of the electrolyte in the solution increases from 0 to 2.5 M. The dehydration of the polar shell was correlated to the average value of the cation hydration number calculated from literature data; the greater the cation hydration number, the greater the dehydration for the same increase in electrolyte concentration. Also, it was shown that the cloud point is strongly correlated to the hydration of the polar shell.

| S-EPMC2790041 | biostudies-literature

1-Anilinonaphthalene-8-sulphonate, a fluorescent conformational probe for glutamate dehydrogenase.

Project description:1. The interaction of 1-anilinonaphthalene-8-sulphonate with ox liver glutamate dehydrogenase was examined. 2. The fluorescence of the dye is enhanced 100-fold on binding. 3. A further enhancement is observed when NADH and GTP are added to the enzyme. 4. By using this property of the dye to measure conformational equilibria in the enzyme the effects of coenzyme, inhibitors, enzyme concentration, ionic strength and pH on the allosteric transitions were studied. 5. GTP and NADH interact with the enzyme in a heterotropic manner. 6. The rate of the structural transition brought about by GTP and NADH is biphasic with half-lives of 34 and 200msec. 7. The relation of these observations to regulatory mechanisms is discussed.

| S-EPMC1184868 | biostudies-other

Phosphorylation of synaptic-membrane proteins from ox cerebral cortex in vitro. Partition of substrates and protein kinase activities with triton X-100.

Project description:Synaptic-membrane fragments from ox cerebral cortex contain basal and cyclic AMP-stimulated protein kinase activity catalysing the phosphorylation of endogenous substrates. Extraction of membrane fragments with Triton X-100 solubilized less than 20% of the kinase activity and left the major part of the endogenous substrates in the insoluble fraction.

| S-EPMC1163908 | biostudies-other

Carbohydrate-containing Triton X-100 analogues for membrane protein solubilization and stabilization.

Project description:Membrane protein manipulation is a challenging task owing to limited tertiary and quaternary structural stability once the protein has been removed from a lipid bilayer. Such instability can be overcome by embedding membrane proteins in detergent micelles formed from amphiphiles with carefully tuned properties. This study introduces a class of easy-to-synthesize amphiphiles, which are designated CGT (Chae's Glyco-Triton) detergents. Some of the agents are well suited for membrane protein solubilization and stabilization.

| S-EPMC3593792 | biostudies-literature

Iron-dependent binding of 8-anilinonaphthalene-1-sulphonate by both lactoferrin and transferrin.

Project description:Fluorescence of 8-anilinonaphthalene-1-sulphonate is enhanced by both lactoferrin and transferrin. The enhancement is relatively low between pH 4 and 8, and high at below pH 4. At physiological pH the fluorescence enhancement is higher with the iron-deprived than with the iron-saturated proteins. Binding of iron by lactoferrin is associated with lowering affinity for the dye.

| S-EPMC1163837 | biostudies-other

The binding of 8-anilinonaphthalene-1-sulphonate to cytoplasmic aspartate aminotransferase from pig heart.

Project description:Anilinonaphthalenesulphonate binds to cytoplasmic aspartate aminotransferase with high affinity (Kd about 10 muM) and with a stoicheiometry of one molecule per dimer. It is not displaced by aliphatic or aromatic dicarboxylate substrate analogues. The enzyme is believed to be a symmetrical dimer with identical subunits; it can evidently function asymmetrically in binding anilinonaphthalenesulphonate.

| S-EPMC1165195 | biostudies-other

Identification of compendial nonionic detergents for the replacement of Triton X-100 in bioprocessing.

Project description:We have systematically investigated six compendial nonionic detergents as potential replacements for Triton ×-100 in bioprocessing applications. Use of compendial raw materials in cGMP bioprocessing is advantageous for a variety of reasons including material specifications developed to meet stringent pharmaceutical product quality requirements, regulatory familiarity and comfort, and availability from vendors experienced supplying the biopharmaceutical industry. We first examine material properties of the detergents themselves including melting point and viscosity. Process performance and product contact in real-world bioprocess applications are then investigated. Lastly, we test the detergents in virus inactivation (VI) experiments with recombinant proteins and adeno-associated virus. Two of the detergents tested, PEG 9 Lauryl Ether and PEG 6 Caprylic/Capric Glycerides, showed favorable properties that make them attractive for use as potential Triton X-100 replacements. Process performance testing indicated negligible impact of the detergents on product yield, purity, and activity compared to a control with no detergent. Importantly, both PEG 9 Lauryl Ether and PEG 6 Caprylic/Capric Glycerides demonstrated very fast VI kinetics with complete inactivation of XMuLV observed in less than 1 min at a target 1% detergent concentration. Potential advantages and disadvantages of both candidate detergents for use in cGMP bioprocessing are summarized and discussed.

| S-EPMC9285696 | biostudies-literature

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data