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The Effect of Residual Triton X-100 on Structural Stability and Infection Activity of Adenovirus Particles

ABSTRACT: To ensure the high purity and biological activity of the adenovirus vector to be used for clinical applications, a stable and linearly scalable preparation method is highly imperative. During the adenovirus-harvesting process, the Triton X-100-based lysis method possesses the advantages of higher efficiency as well as easier linearization and amplification. Most Triton X-100 can be removed from the adenovirus sample by chromatographic purification. However, there is no report that a small amount of residual Triton X-100, present in adenovirus sample, can affect the particle integrity, infectivity, and structure of adenoviruses. Here, we found that although residual Triton X-100 affected the short-term stability, purity, infectivity, and structure of adenoviruses at 37°C, it did not hamper these properties of adenoviruses at 4°C. This study suggests that although the Triton X-100-based lysis method is a simple, efficient, and easy-to-scale process for lysing host cells to release the adenovirus, the storage conditions of adenovirus products must be taken into consideration. Graphical Abstract The Triton X-100-based lysis method is a simple, efficient, and easy-to-scale process for lysing host cells to release the adenovirus. However, Ping Cheng and colleagues reported the residual Triton X-100 in purified adenovirus had different effects on the structural stability and infection activity of adenovirus particles under different temperature storage conditions.

SUBMITTER: Ma J

PROVIDER: S-EPMC7490641 | biostudies-literature | 2020 Aug

REPOSITORIES: biostudies-literature

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