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Dodecyl sulphate/polyacrylamide-gel electrophoresis at low pH values and low temperatures.

ABSTRACT: A simple method is described for dodecyl sulphate/polyacrylamide-gel electrophoresis of pH- and temperature-labile biological intermediates. The method is based on a catalyst system that works at temperatures of 2--4 degrees C and pH values of 2--4 and an appropriate buffer system containing Li+ or Tris [CH2OH--C(CH2OH)2--NH3+] instead of Na+. This system does not lead to the precipitation of 1% dodecyl sulphate.

SUBMITTER: Lichtner R

PROVIDER: S-EPMC1161216 | biostudies-other | 1979 Sep

REPOSITORIES: biostudies-other

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Highly purified agarose as stacking gel in sodium dodecyl sulphate/polyacrylamide-gel electrophoresis.

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| S-EPMC1162698 | biostudies-other

Differentiation of Bartonella species by a microimmunofluorescence assay, sodium dodecyl sulfate-polyacrylamide gel electrophoresis, and Western immunoblotting.

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| S-EPMC95924 | biostudies-literature

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