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A method for the preparation of low-pH dodecyl sulphate/polyacrylamide-gradient gels.

ABSTRACT: 1. A low-pH lithium dodecyl sulphate/polyacrylamide-gradient slab-gel system, suitable for electrophoresis, is described, and the migration properties of standard proteins are compared on this and conventional high-pH gels. 2. Cytochrome oxidase may be partially resolved into its component polypeptides. The order of migration of these is, however, dependent on the pH of the gel system.

SUBMITTER: Jones GD

PROVIDER: S-EPMC1162698 | biostudies-other | 1981 Mar

REPOSITORIES: biostudies-other

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Dodecyl sulphate/polyacrylamide-gel electrophoresis at low pH values and low temperatures.

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