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A simple and fast method for the isolation of basolateral plasma membranes from rat small-intestinal epithelial cells.


ABSTRACT: A method was developed for the analytical and preparative isolation of basolateral plasma membranes from rat small intestine. They were separated on a self-orientating Percoll (modified colloidal silica) gradient starting with a heavy microsomal-membrane fraction and involving centrifugation at 48,000 g for 1 h. (Na+ + K+)-stimulated ATPase activity, used as a marker enzyme for the basolateral plasma membrane, is enriched 20-fold compared with that found in the homogenate of isolated intestinal epithelial cells.

SUBMITTER: Scalera V 

PROVIDER: S-EPMC1161517 | biostudies-other | 1980 Jan

REPOSITORIES: biostudies-other

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