Dataset Information

LogSpin: a simple, economical and fast method for RNA isolation from infected or healthy plants and other eukaryotic tissues.

ABSTRACT:

Background

Rapid RNA extraction is commonly performed with commercial kits, which are very expensive and can involve toxic reagents. Most of these kits can be used with healthy plant tissues, but do not produce consistently high-quality RNA from necrotic fungus-infected tissues or fungal mycelium.

Findings

We report on the development of a rapid and relatively inexpensive method for total RNA extraction from plants and fungus-infected tissues, as well as from insects and fungi, based on guanidine hydrochloride buffer and common DNA extraction columns originally used for the extraction and purification of plasmids and cosmids.

Conclusions

The proposed method can be used reproducibly for RNA isolation from a variety of plant species. It can also be used with infected plant tissue and fungal mycelia, which are typically recalcitrant to standard nucleic acid extraction procedures.

SUBMITTER: Yaffe H

PROVIDER: S-EPMC3282632 | biostudies-literature | 2012 Jan

REPOSITORIES: biostudies-literature

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Publications

LogSpin: a simple, economical and fast method for RNA isolation from infected or healthy plants and other eukaryotic tissues.

Yaffe Hila H Buxdorf Kobi K Shapira Illil I Ein-Gedi Shachaf S Moyal-Ben Zvi Michal M Fridman Eyal E Moshelion Menachem M Levy Maggie M

BMC research notes 20120119

<h4>Background</h4>Rapid RNA extraction is commonly performed with commercial kits, which are very expensive and can involve toxic reagents. Most of these kits can be used with healthy plant tissues, but do not produce consistently high-quality RNA from necrotic fungus-infected tissues or fungal mycelium.<h4>Findings</h4>We report on the development of a rapid and relatively inexpensive method for total RNA extraction from plants and fungus-infected tissues, as well as from insects and fungi, ba ...[more]

PMID: 22260178

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Dataset Information

LogSpin: a simple, economical and fast method for RNA isolation from infected or healthy plants and other eukaryotic tissues.

Background

Findings

Conclusions

Publications

LogSpin: a simple, economical and fast method for RNA isolation from infected or healthy plants and other eukaryotic tissues.

Similar Datasets

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