Unknown

Dataset Information

0

Isolation and characterization of rat plasma fibronectin.


ABSTRACT: Rat plasma fibronectin has been isolated and characterized and monospecific antibodies were prepared to it. Two components of fresh rat plasma (in the presence of proteinase inhibitors) bound to a gelatin-Sepharose affinity column. One protein was eluted with 4.0 M-urea and was identified as fibronectin. Another protein was eluted from the gelatin-Sepharose column with 8.0 M-urea and was identified as a 70 000-Mr collagen-binding molecule. This 70 000-Mr fragment was found to be a normal constituent of blood plasma, and its presence did not represent a proteolytic degradation product formed during isolation. The antibodies prepared against rat fibronectin only weakly cross-reacted with plasma fibronectins of chicken, horse and human. These studies shed light on the metabolic interrelationships between fibronectin and other collagen-binding molecules.

SUBMITTER: Weiss RE 

PROVIDER: S-EPMC1163163 | biostudies-other | 1981 Sep

REPOSITORIES: biostudies-other

Similar Datasets

| S-EPMC1137050 | biostudies-other
| S-EPMC1162272 | biostudies-other
| S-EPMC6801590 | biostudies-literature
| S-EPMC4929849 | biostudies-literature
| S-EPMC4503726 | biostudies-literature
| S-EPMC90686 | biostudies-literature
| S-EPMC1161475 | biostudies-other
| S-EPMC3471917 | biostudies-literature
| S-EPMC2827351 | biostudies-literature
| S-EPMC6630935 | biostudies-literature