Unknown

Dataset Information

0

A sensitive method for measuring protein turnover based on the measurement of 2-3H-labelled amino acids in protein.


ABSTRACT: A method for measuring the rate of protein degradation is described. The method measures the change in 2-3H content of protein with time by racemization of the protein hydrolysate with acetic anhydride. The 3H on C-2 of amino acids is stable in proteins but becomes labile, owing to the action of transaminases, once the amino acids are released by proteolysis. The specific measurement of 2-3H in amino acids largely overcomes problems due to compartmentation and isotope recycling and evidence to support this claim is presented. Values for the half-life of Lemna minor (duckweed) protein determined by the new method are compared with values obtained by other methods.

SUBMITTER: Humphrey TJ 

PROVIDER: S-EPMC1163789 | biostudies-other | 1976 Jun

REPOSITORIES: biostudies-other

Similar Datasets

2010-05-27 | E-GEOD-19788 | biostudies-arrayexpress
2010-05-27 | GSE19788 | GEO
| S-EPMC1165512 | biostudies-other
2021-03-04 | MSV000086999 | MassIVE
| S-EPMC1163210 | biostudies-other
| S-EPMC2721084 | biostudies-other
| S-EPMC10245746 | biostudies-literature
| S-EPMC1131075 | biostudies-other
| S-EPMC4417145 | biostudies-literature
| S-EPMC4365669 | biostudies-literature