Genomics

Dataset Information

0

CATCH-IT method for measuring nucleosome turnover


ABSTRACT: Direct measurement of nucleosome turnover dynamics by using co-translational incorporation of the methionine (Met) surrogate azidohomoalaine (Aha) into proteins and subsequent ligation of biotin to Aha-containing proteins through the [3+2] cycloaddition reaction between the azide group of Aha and an alkyne linked to biotin. To measure turnover rates, we treat cells briefly with Aha, couple biotin to nucleosomes containing newly incorporated histones, affinity purify with strepavidin, wash stringently to remove non-histone proteins and H2A/H2B dimers, and analyze the affinity-purified DNA using tiling microarrays. We call this strategy 'CATCH-IT' for Covalent Attachment of Tags to Capture Histones and Identify Turnover. Keywords: Chromatin affinity-purification on microarray

ORGANISM(S): Drosophila melanogaster

PROVIDER: GSE19788 | GEO | 2010/05/27

SECONDARY ACCESSION(S): PRJNA121809

REPOSITORIES: GEO

Dataset's files

Source:
Action DRS
Other
Items per page:
1 - 1 of 1

Similar Datasets

2010-05-27 | E-GEOD-19788 | biostudies-arrayexpress
2021-04-21 | GSE153253 | GEO
2020-12-31 | GSE132099 | GEO
2024-06-17 | GSE260451 | GEO
2020-10-12 | GSE147238 | GEO
2023-04-28 | PXD027600 | Pride
2023-11-27 | PXD046934 | Pride
2024-07-25 | PXD050770 | Pride
2024-01-12 | PXD043402 | Pride
2023-02-11 | MSV000091255 | MassIVE