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A simple method of characterizing mitochondrial ribonucleic acid.

ABSTRACT: A simple method of isolating and characterizing RNA from L-cell mitochondria is described. The mitochondrial fraction is lysed by sodium dodecyl sulphate, and the RNA fractionated by sucrose-density-gradient centrifugation. The efficacy of proteinase K in preventing ribonuclease activity is also demonstrated.

SUBMITTER: Haldar D 

PROVIDER: S-EPMC1165492 | biostudies-other | 1975 Jun

REPOSITORIES: biostudies-other

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