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Incorporation of label from D- -hydroxy( 14 C)butyrate and (3- 14 C)acetoacetate into amino acids in rat brain in vivo.

ABSTRACT: The metabolism of ketone bodies by rat brain was studied in vivo. Rats starved for 48h were given either d-beta-hydroxy[3-(14)C]butyrate or [3-(14)C]acetoacetate by intravenous injection and killed after 3 or 10min. Total radioactivity in the acid-soluble material of the brain and the specific radioactivities of the brain amino acids glutamate, glutamine, aspartate and gamma-aminobutyrate were determined. A group of fed animals were also given d-beta-hydroxy[3-(14)C]butyrate. In the brains of all animals (14)C was present in the acid-soluble material and the specific radioactivity of glutamate was greater than that of glutamine.

SUBMITTER: Cremer JE

PROVIDER: S-EPMC1176756 | biostudies-other | 1971 Apr

REPOSITORIES: biostudies-other

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