Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Double-helical character of ribonucleic acid from virus-like particles found in Penicillium chrysogenum.

ABSTRACT: 1. RNA was isolated from virus-like particles found in Penicillium chrysogenum and resolved into two fractions by gel filtration through agarose columns. 2. Fraction 1 was excluded and had the following properties: 50.9% G+C [AMP 0.246, UMP 0.246, CMP 0.252, GMP 0.255 (mole fraction)]; mol.wt. about 1.2x10(6) daltons; s(20,w) 12.3S and ;melting' temperature about 100 degrees C (solvent 0.15m-sodium chloride-0.015m-sodium citrate pH7.2); optical rotation [alpha](max.) 6000 degrees at 278nm; circular dichroism (epsilon(L)-epsilon(R))(max.)=8.181mol(-1) cm(-1) at 260nm. 3. Properties of fraction 2 include 37.8% G+C [AMP 0.313, UMP 0.312, CMP 0.186, GMP 0.189 (mole fraction)]; mol.wt. about 140000 daltons; s(20,w) 7.3S, T(m) about 85 degrees C (solvent 0.15m-sodium chloride-0.015m-sodium citrate, pH7.2); optical rotation [alpha](max.) 6000 degrees at 278nm; circular dichroism (epsilon(L)-epsilon(R))(max.)=8.241mol(-1) cm(-1) at 260nm. 4. The properties of both fractions were consistent with a double-helical conformation.

SUBMITTER: Cox RA

PROVIDER: S-EPMC1179636 | biostudies-other | 1970 Dec

REPOSITORIES: biostudies-other

ACCESS DATA

Json Xml

Similar Datasets

Thermal denaturation in acidic solutions of double-helical ribonucleic acid from virus-like particles found in Penicillium chrysogenum. A spectrophotometric study.

Project description:1. Two species of double-helical RNA isolated from mycelium of Penicillium chrysogenum were titrated with acid at 25 degrees C and 95 degrees C (solvent 0.1m-sodium phosphate buffer). At 25 degrees C denaturation occurred at about pH3. At 95 degrees C in the denatured form cytosine residues titrated as a simple monobasic acid of pK3.9 compared with pK approximately 2.5 for the native form at 25 degrees C. 2. On thermal denaturation in neutral and acidic solutions one species of RNA (38% rG.rC) ;melted' in three distinct stages, equivalent to a mixture of three species, namely one of about 25% rG.rC, another of about 33% rG.rC and a third of about 46% rG.rC: the relative proportions were 0.25:0.35:0.40. 3. On thermal denaturation in acidic solutions the increase in the fraction of ionized cytosine residues concomitant with the ;melting' of rG.rC base pair also affects the spectrum especially at 280nm and serves to enhance the contribution of rG.rC base pairs at this wavelength. The increment in epsilon((P)) at 280nm on ;melting' an rG.rC base pair approaches 53501.mol(-1).cm(-1) depending on pH, compared with 33501.mol(-1).cm(-1) at pH7. In contrast epsilon((P)) at 280nm is scarcely affected by ;melting' rA.rU base pairs or by the protonization of adenine residues. 4. Changes in the spectrum of Escherichia coli rRNA on denaturation in acidic solutions were studied to yield the mole fractions of rA.rU and rG.rC base pairs ;melting' at particular pH values.

| S-EPMC1178103 | biostudies-other

How does hydroxyl introduction influence the double helical structure: the stabilization of an altritol nucleic acid:ribonucleic acid duplex.

Project description:Altritol nucleic acids (ANAs) are a promising new tool in the development of artificial small interfering ribonucleic acids (siRNAs) for therapeutical applications. To mimic the siRNA:messenger RNA (mRNA) interactions, the crystal structure of the ANA:RNA construct a(CCGUAAUGCC-P):r(GGCAUUACGG) was determined to 1.96 Å resolution which revealed the hybrid to form an A-type helix. As this A-form is a major requirement in the RNAi process, this crystal structure confirms the potential of altritol-modified siRNAs. Moreover, in the ANA strands, a new type of intrastrand interactions was found between the O2' hydroxyl group of one residue and the sugar ring O4' atom of the next residue. These interactions were further investigated by quantum chemical methods. Besides hydration effects, these intrastrand hydrogen bonds may also contribute to the stability of ANA:RNA duplexes.

| S-EPMC3424580 | biostudies-literature

Fractionation of nucleic acids from Penicillium chrysogenum and associated ribonucleic acid viruses by selective exclusion and retention in agarose gels.

Project description:Double-stranded nucleic acids from a strain of Penicillium chrysogenum containing RNA viruses were isolated by agarose-gel filtration, and separated into DNA and double-stranded RNA fractions by agarose-gel chromatography in 2.5m-NaCl. The DNA fraction contained less than 1% alkali-labile polynucleotides, and sedimented homogeneously at 8-10S in alkaline sucrose gradients. In CsCl gradients it tended to band in the density region of 1.66-1.72g/ml. It had a ;melting' temperature (T(m)) of 75 degrees C in 0.015m-NaCl-0.0015m-trisodium citrate, corresponding to 51.5mol% of G+C. The double-stranded RNA fraction did not contain detectable DNA. It could not band in CsCl up to a density of 1.78g/ml, and mainly consisted of a 14-15S RNA species with a T(m) of 88.5 degrees C in the above solvent, and a G+C content of 49.3 mol%.

| S-EPMC1166262 | biostudies-other

Penicillium chrysogenum

Project description:Characterization of the Ku70 homologue HdfA deletion in Penicillium chrysogenum

| PRJNA110889 | ENA

Penicillium chrysogenum

Project description:Molecular analysis of a microbial strain improvement paradigm

| PRJNA103789 | ENA

Penicillium chrysogenum

Project description:Engineering of Penicillium chrysogenum for fermentative production of a novel carbamoylated cephem antibiotic precursor

| PRJNA112879 | ENA

Penicillium chrysogenum

Project description:Penicillium chrysogenum mRNA-seq

| PRJNA353579 | ENA

Penicillium chrysogenum

Project description:Functional characterization of a P. chrysogenum mutanase identified by co-cultivation with Bacillus subtilis.

| PRJNA231564 | ENA

Penicillium chrysogenum

Project description:Degeneration of penicillin production in ethanol-limited chemostat cultivation of Penicillium chrysogenum: A systems biology approach

| PRJNA132945 | ENA

Penicillium chrysogenum

Project description:ΔMAT1-1-1 (Pc20g07800) vs. P2niaD18 (wild type)

| PRJNA284633 | ENA