Ontology highlight
ABSTRACT:
SUBMITTER: Moriyama K
PROVIDER: S-EPMC1188086 | biostudies-other | 2005
REPOSITORIES: biostudies-other
Nucleic acids research 20050819 15
Direct site-specific biotinylation of RNA molecules was achieved by specific transcription mediated by unnatural base pairs. Unnatural base pairs between 2-amino-6-(2-thienyl)purine (denoted by s) and 2-oxo(1H)pyridine (denoted by y), or 2-amino-6-(2-thiazolyl)purine (denoted as v) and y specifically function in T7 transcription. Using these unnatural base pairs, the substrate of biotinylated-y (Bio-yTP) was selectively incorporated into RNA, opposite s or v in the DNA templates, by T7 RNA polym ...[more]