Ontology highlight
ABSTRACT:
SUBMITTER: Sato M
PROVIDER: S-EPMC1253562 | biostudies-other | 2005 Oct
REPOSITORIES: biostudies-other
Sato Moritoshi M Hida Naoki N Umezawa Yoshio Y
Proceedings of the National Academy of Sciences of the United States of America 20050921 41
Nitric oxide (NO) is a small uncharged free radical that is involved in diverse physiological and pathophysiological mechanisms. NO is generated by three isoforms of NO synthase, endothelial, neuronal, and inducible ones. When generated in vascular endothelial cells, NO plays a key role in vascular tone regulation, in particular. Here, we describe an amplifier-coupled fluorescent indicator for NO to visualize physiological nanomolar dynamics of NO in living cells (detection limit of 0.1 nM). Thi ...[more]