Ontology highlight
ABSTRACT:
SUBMITTER: Bebear CM
PROVIDER: S-EPMC163700 | biostudies-other | 1997 Feb
REPOSITORIES: biostudies-other
Bebear C M CM Bové J M JM Bebear C C Renaudin J J
Antimicrobial agents and chemotherapy 19970201 2
Fluoroquinolone-resistant mutants of Mycoplasma hominis were selected in vitro from the PG21 susceptible reference strain either by multistep selection on increasing concentrations of various fluoroquinolones or by one-step selection on agar medium with ofloxacin. The quinolone resistance-determining regions (QRDR) of the structural genes encoding the A and b subunits of DNA gyrase were amplified by PCR, and the nucleotide sequences of eight multistep-selected resistant strains were compared to ...[more]