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Isolation of a beta-tubulin gene from Fusarium moniliforme that confers cold-sensitive benomyl resistance.

ABSTRACT: A beta-tubulin gene from a UV-irradiated benomyl-resistant mutant of Fusarium moniliforme was isolated, cloned, and sequenced. The gene encodes a 446-amino-acid polypeptide with homology to other fungal beta-tubulins. RNA blot analysis showed expression of the gene during vegetative growth and conidial germination but no expression during conidiation. A point mutation, which likely confers benomyl resistance, has been identified in the cloned gene; this mutation results in a single amino acid substitution of asparagine for tyrosine at position 50. Expression of benomyl resistance in the mutant was also cold sensitive. Sexual crosses betweeen the mutant and a wild-type strain indicated cosegregation of benomyl resistance and cold sensitivity.

SUBMITTER: Yan K

PROVIDER: S-EPMC168094 | biostudies-other | 1996 Aug

REPOSITORIES: biostudies-other

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Isolation of a beta-tubulin gene from Fusarium moniliforme that confers cold-sensitive benomyl resistance.

Yan K K Dickman M B MB

Applied and environmental microbiology 19960801 8

A beta-tubulin gene from a UV-irradiated benomyl-resistant mutant of Fusarium moniliforme was isolated, cloned, and sequenced. The gene encodes a 446-amino-acid polypeptide with homology to other fungal beta-tubulins. RNA blot analysis showed expression of the gene during vegetative growth and conidial germination but no expression during conidiation. A point mutation, which likely confers benomyl resistance, has been identified in the cloned gene; this mutation results in a single amino acid su ...[more]

PMID: 8702300

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