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Identification and characterization of the pckA gene from Staphylococcus aureus.


ABSTRACT: The Staphylococcus aureus pckA gene was identified and characterized. A pckA mutant lacked detectable phosphoenolpyruvate carboxykinase activity and grew poorly in the absence of glucose. Both enzymatic activity and pckA promoter activity in wild-type cells grown in the absence of glucose were at least 22-fold greater than activities in cells grown in the presence of glucose.

SUBMITTER: Scovill WH 

PROVIDER: S-EPMC178095 | biostudies-other | 1996 Jun

REPOSITORIES: biostudies-other

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Identification and characterization of the pckA gene from Staphylococcus aureus.

Scovill W H WH   Schreier H J HJ   Bayles K W KW  

Journal of bacteriology 19960601 11


The Staphylococcus aureus pckA gene was identified and characterized. A pckA mutant lacked detectable phosphoenolpyruvate carboxykinase activity and grew poorly in the absence of glucose. Both enzymatic activity and pckA promoter activity in wild-type cells grown in the absence of glucose were at least 22-fold greater than activities in cells grown in the presence of glucose. ...[more]

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