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The uvrB gene of Pseudomonas aeruginosa is not DNA damage inducible.

ABSTRACT: The uvrB gene of Pseudomonas aeruginosa has been isolated from a genomic library by complementation of an Escherichia coli uvrB mutant. The complete nucleotide sequence of P. aeruginosa uvrB consists of 2,013 bp, encoding a polypeptide of 670 amino acids. A P. aeruginosa SOS consensus region, which functions as a binding site for the LexA repressor molecule, is not present in the upstream region of the uvrB gene isolated. By transcriptional fusions with a reporter gene, it has been demonstrated that, contrary to what happens with the homologous gene of E. coli, the P. aeruginosa uvrB gene is not DNA damage inducible. Nevertheless, the UvrB protein must be functional in P. aeruginosa cells because a uvrB-defective mutant is extremely sensitive to UV radiation.

SUBMITTER: Rivera E

PROVIDER: S-EPMC178385 | biostudies-other | 1996 Sep

REPOSITORIES: biostudies-other

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The uvrB gene of Pseudomonas aeruginosa is not DNA damage inducible.

Rivera E E Vila L L Barbé J J

Journal of bacteriology 19960901 18

The uvrB gene of Pseudomonas aeruginosa has been isolated from a genomic library by complementation of an Escherichia coli uvrB mutant. The complete nucleotide sequence of P. aeruginosa uvrB consists of 2,013 bp, encoding a polypeptide of 670 amino acids. A P. aeruginosa SOS consensus region, which functions as a binding site for the LexA repressor molecule, is not present in the upstream region of the uvrB gene isolated. By transcriptional fusions with a reporter gene, it has been demonstrated ...[more]

PMID: 8808952

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Project description:BackgroundThe bacterium Pseudomonas aeruginosa is capable of three types of motilities: swimming, twitching and swarming. The latter is characterized by a fast and coordinated group movement over a semi-solid surface resulting from intercellular interactions and morphological differentiation. A striking feature of swarming motility is the complex fractal-like patterns displayed by migrating bacteria while they move away from their inoculation point. This type of group behaviour is still poorly understood and its characterization provides important information on bacterial structured communities such as biofilms. Using GeneChip® Affymetrix microarrays, we obtained the transcriptomic profiles of both bacterial populations located at the tip of migrating tendrils and swarm center of swarming colonies and compared these profiles to that of a bacterial control population grown on the same media but solidified to not allow swarming motility.ResultsMicroarray raw data were corrected for background noise with the RMA algorithm and quantile normalized. Differentially expressed genes between the three conditions were selected using a threshold of 1.5 log2-fold, which gave a total of 378 selected genes (6.3% of the predicted open reading frames of strain PA14). Major shifts in gene expression patterns are observed in each growth conditions, highlighting the presence of distinct bacterial subpopulations within a swarming colony (tendril tips vs. swarm center). Unexpectedly, microarrays expression data reveal that a minority of genes are up-regulated in tendril tip populations. Among them, we found energy metabolism, ribosomal protein and transport of small molecules related genes. On the other hand, many well-known virulence factors genes were globally repressed in tendril tip cells. Swarm center cells are distinct and appear to be under oxidative and copper stress responses.ConclusionsResults reported in this study show that, as opposed to swarm center cells, tendril tip populations of a swarming colony displays general down-regulation of genes associated with virulence and up-regulation of genes involved in energy metabolism. These results allow us to propose a model where tendril tip cells function as «scouts» whose main purpose is to rapidly spread on uncolonized surfaces while swarm center population are in a state allowing a permanent settlement of the colonized area (biofilm-like).

Dataset Information

The uvrB gene of Pseudomonas aeruginosa is not DNA damage inducible.

Publications

The uvrB gene of Pseudomonas aeruginosa is not DNA damage inducible.

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