Ontology highlight
ABSTRACT:
SUBMITTER: Jun Y
PROVIDER: S-EPMC1941832 | biostudies-other | 2007 Aug
REPOSITORIES: biostudies-other
Jun Youngsoo Y Wickner William W
Proceedings of the National Academy of Sciences of the United States of America 20070730 32
Membrane fusion entails organelle docking and subsequent mixing of membrane bilayers and luminal compartments. We now present an in vitro assay of fusion, using yeast vacuoles bearing domains of either Fos or Jun fused to complementary halves of beta-lactamase. Upon fusion, these proteins associate to yield beta-lactamase activity. This assay complements the standard fusion assay (activation of pro-Pho8p in protease-deficient vacuoles by proteases from pho8Delta vacuoles). Both the beta-lactamas ...[more]