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Recognition of chimeric small-subunit ribosomal DNAs composed of genes from uncultivated microorganisms.


ABSTRACT: When PCR was used to recover small-subunit (SSU) rRNA genes from a hot spring cyanobacterial mat community, chimeric SSU rRNA sequences which exhibited little or no secondary structural abnormality were recovered. They were revealed as chimeras of SSU rRNA genes of uncultivated species through separate phylogenetic analysis of short sequence domains.

SUBMITTER: Kopczynski ED 

PROVIDER: S-EPMC201378 | biostudies-other | 1994 Feb

REPOSITORIES: biostudies-other

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Recognition of chimeric small-subunit ribosomal DNAs composed of genes from uncultivated microorganisms.

Kopczynski E D ED   Bateson M M MM   Ward D M DM  

Applied and environmental microbiology 19940201 2


When PCR was used to recover small-subunit (SSU) rRNA genes from a hot spring cyanobacterial mat community, chimeric SSU rRNA sequences which exhibited little or no secondary structural abnormality were recovered. They were revealed as chimeras of SSU rRNA genes of uncultivated species through separate phylogenetic analysis of short sequence domains. ...[more]

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