Unknown

Dataset Information

0

Molecular analysis of the rolling-circle replicating plasmid pA1 of Lactobacillus plantarum A112.


ABSTRACT: Lactobacillus plantarum A112 has four different plasmids. Plus-origin-specific probes were used to determine that the smallest, cryptic plasmid, pA1 (2,820 bp), showed homology to the pE194 plasmid family. This subclass of plasmids uses the rolling-circle mode of replication. Subsequent analysis of plasmid pA1 demonstrated that it generates single-stranded DNA intermediates, and sequence analysis revealed that it contains three putative open reading frames (ORFs): ORF1, ORF2, and ORF3, which could encode proteins designated RepA (47 amino acids [aa]) and RepB (196 aa) and a protein of 103 aa, respectively. Two of these proteins, RepA (5.6 kDa) and RepB (26 kDa), were identified in in vitro transcription translation assays. The RepA protein contains a characteristic alpha-helix-turn-alpha-helix motif typical of DNA-binding proteins that act as DNA-binding repressors. The RepB protein shows a significant similarity with replication initiation proteins of the pE194 family of plasmids that use the rolling-circle mode of replication. Plasmid pA1 is able to replicate in Escherichia coli and Lactobacillus lactis subsp. lactis as well as in other L. plantarum strains.

SUBMITTER: Vujcic M 

PROVIDER: S-EPMC202090 | biostudies-other | 1993 Jan

REPOSITORIES: biostudies-other

altmetric image

Publications

Molecular analysis of the rolling-circle replicating plasmid pA1 of Lactobacillus plantarum A112.

Vujcic M M   Topisirovic L L  

Applied and environmental microbiology 19930101 1


Lactobacillus plantarum A112 has four different plasmids. Plus-origin-specific probes were used to determine that the smallest, cryptic plasmid, pA1 (2,820 bp), showed homology to the pE194 plasmid family. This subclass of plasmids uses the rolling-circle mode of replication. Subsequent analysis of plasmid pA1 demonstrated that it generates single-stranded DNA intermediates, and sequence analysis revealed that it contains three putative open reading frames (ORFs): ORF1, ORF2, and ORF3, which cou  ...[more]

Similar Datasets

| S-EPMC1563867 | biostudies-literature
| S-EPMC95193 | biostudies-literature
| S-EPMC6687099 | biostudies-literature
| S-EPMC4297665 | biostudies-literature
| S-EPMC7103276 | biostudies-literature
| S-EPMC3792671 | biostudies-literature
| S-EPMC5568012 | biostudies-other
| S-EPMC3340939 | biostudies-literature
| S-EPMC37501 | biostudies-literature
| S-EPMC520868 | biostudies-literature