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Nucleotide sequence of the gene for cholesterol oxidase from a Streptomyces sp.

ABSTRACT: The nucleotide sequence of a 2.1-kilobase-pair fragment containing the Streptomyces choA gene, which codes a secreted cholesterol oxidase, was determined. A single open reading frame encodes a mature cholesterol oxidase of 504 amino acids, with a calculated Mr of 54,913. The leader peptides extend over 42 amino acids and have the characteristics of a signal sequence, including basic amino acids near the amino terminus and a hydrophobic core near the signal cleavage site. Analyses of the total amino acid composition and amino acid sequencing of the first 21 amino acids from the N terminus of the purified extracellular enzyme agree with the values deduced from nucleotide sequencing data.

SUBMITTER: Ishizaki T

PROVIDER: S-EPMC209631 | biostudies-other | 1989 Jan

REPOSITORIES: biostudies-other

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Nucleotide sequence of the gene for cholesterol oxidase from a Streptomyces sp.

Ishizaki T T Hirayama N N Shinkawa H H Nimi O O Murooka Y Y

Journal of bacteriology 19890101 1

The nucleotide sequence of a 2.1-kilobase-pair fragment containing the Streptomyces choA gene, which codes a secreted cholesterol oxidase, was determined. A single open reading frame encodes a mature cholesterol oxidase of 504 amino acids, with a calculated Mr of 54,913. The leader peptides extend over 42 amino acids and have the characteristics of a signal sequence, including basic amino acids near the amino terminus and a hydrophobic core near the signal cleavage site. Analyses of the total am ...[more]

PMID: 2914858

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