Ontology highlight
ABSTRACT:
SUBMITTER: Korlach J
PROVIDER: S-EPMC2234111 | biostudies-other | 2008 Jan
REPOSITORIES: biostudies-other
Proceedings of the National Academy of Sciences of the United States of America 20080123 4
Optical nanostructures have enabled the creation of subdiffraction detection volumes for single-molecule fluorescence microscopy. Their applicability is extended by the ability to place molecules in the confined observation volume without interfering with their biological function. Here, we demonstrate that processive DNA synthesis thousands of bases in length was carried out by individual DNA polymerase molecules immobilized in the observation volumes of zero-mode waveguides (ZMWs) in high-dens ...[more]