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Phage display of functional, full-length human and viral membrane proteins.

ABSTRACT: Phage display of protein and peptide libraries offers a powerful technology for the selection and isolation of ligands and receptors. To date, the technique has been considered limited to soluble, non-membrane proteins. We report two examples of phage display of full-length, folded and functional membrane proteins. Consistent display required the recently reported KO7(+) helper phage. The two proteins, full-length caveolin-1 and HIV gp41, display well on the surface of the phage, and maintain their binding activities as shown by in vitro assays.

SUBMITTER: Majumdar S

PROVIDER: S-EPMC2597205 | biostudies-other | 2008 Nov

REPOSITORIES: biostudies-other

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Phage display of functional, full-length human and viral membrane proteins.

Majumdar Sudipta S Hajduczki Agnes A Mendez Aaron S AS Weiss Gregory A GA

Bioorganic & medicinal chemistry letters 20080717 22

Phage display of protein and peptide libraries offers a powerful technology for the selection and isolation of ligands and receptors. To date, the technique has been considered limited to soluble, non-membrane proteins. We report two examples of phage display of full-length, folded and functional membrane proteins. Consistent display required the recently reported KO7(+) helper phage. The two proteins, full-length caveolin-1 and HIV gp41, display well on the surface of the phage, and maintain th ...[more]

PMID: 18667306

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