Isolation of specific DNA fragments of Mycobacterium avium and their possible use in diagnosis.
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ABSTRACT:
SUBMITTER: Thierry D
PROVIDER: S-EPMC262878 | biostudies-other | 1993 May
REPOSITORIES: biostudies-other
Publications
Isolation of specific DNA fragments of Mycobacterium avium and their possible use in diagnosis.
Thierry D D Vincent V V Clément F F Guesdon J L JL
Journal of clinical microbiology 19930501 5
We cloned and sequenced two DNA fragments (DT1 and DT6) from Mycobacterium avium serotype 2 for use in the identification of members of the M. avium-M. intracellulare complex (MAC). Reference strains of MAC belonging to serovars 1 to 28 were examined by using these DNA fragments as probes. The study revealed that the DT6 probe hybridized with DNAs from M. avium strains (serovars 1 to 6, 8 to 11, and 21), while the DT1 probe hybridized with DNAs from serovars 2, 3, 7, 12 to 20, and 23 to 25. DT1- ...[more]
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