Ontology highlight
ABSTRACT:
SUBMITTER: Del Portillo P
PROVIDER: S-EPMC270291 | biostudies-other | 1991 Oct
REPOSITORIES: biostudies-other
Del Portillo P P Murillo L A LA Patarroyo M E ME
Journal of clinical microbiology 19911001 10
In recent work, a species-specific Mycobacterium tuberculosis DNA fragment was cloned and sequenced. On the basis of its nucleotide sequence, two oligonucleotides were synthesized and used as primers for polymerase chain reaction (PCR) amplification. A 396-bp fragment was specifically amplified from the M. tuberculosis genome. No amplification was observed from any of 10 different mycobacterial strains, included those belonging to the M. tuberculosis complex. Neither was this fragment amplified ...[more]