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The functional domain grouping of microtubule associated proteins.

ABSTRACT: Microtubules (MTs), which play crucial roles in normal cell function, are regulated by MT associated proteins (MAPs). Using a combinatorial approach that includes biochemistry, proteomics and bioinformatics, we have recently identified 270 putative MAPs from Drosophila embryos and characterized some of those required for correct progression through mitosis. Here we identify functional groups of these MAPs using a reciprocal hits sequence alignment technique and assign InterPro functional domains to 28 previously uncharacterized proteins. This approach gives insight into the potential functions of MAPs and how their roles may affect MTs.

SUBMITTER: Fisher KH

PROVIDER: S-EPMC2633798 | biostudies-other | 2008

REPOSITORIES: biostudies-other

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The functional domain grouping of microtubule associated proteins.

Fisher Katherine H KH Deane Charlotte M CM Wakefield James G JG

Communicative & integrative biology 20080101 1

Microtubules (MTs), which play crucial roles in normal cell function, are regulated by MT associated proteins (MAPs). Using a combinatorial approach that includes biochemistry, proteomics and bioinformatics, we have recently identified 270 putative MAPs from Drosophila embryos and characterized some of those required for correct progression through mitosis. Here we identify functional groups of these MAPs using a reciprocal hits sequence alignment technique and assign InterPro functional domains ...[more]

PMID: 19704789

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