Ontology highlight
ABSTRACT:
SUBMITTER: Markarian MZ
PROVIDER: S-EPMC2925268 | biostudies-other | 2010 Aug
REPOSITORIES: biostudies-other
Markarian Marie Z MZ Schlenoff Joseph B JB
The journal of physical chemistry. B 20100801 32
The isothermal hybridization of complementary oligonucleotides, 15-mer, 25-mer, 35-mer, and a molecular beacon, was investigated under varying conditions of molecular crowding and ionic strength, using hypochromicity to follow strand pairing and polyethylene glycol as a crowding agent. Thermodynamic analysis of the results revealed the addition of counterions to the oligonucleotide backbones, DeltaPsi, to be dependent on the strand GC content and the molecular crowding. A decrease in DeltaPsi wa ...[more]