Unknown

Dataset Information

0

Dot1 binding induces chromatin rearrangements by histone methylation-dependent and -independent mechanisms.


ABSTRACT: BACKGROUND: Methylation of histone H3 lysine 79 (H3K79) by Dot1 is highly conserved among species and has been associated with both gene repression and activation. To eliminate indirect effects and examine the direct consequences of Dot1 binding and H3K79 methylation, we investigated the effects of targeting Dot1 to different positions in the yeast genome. RESULTS: Targeting Dot1 did not activate transcription at a euchromatic locus. However, chromatin-bound Dot1 derepressed heterochromatin-mediated gene silencing over a considerable distance. Unexpectedly, Dot1-mediated derepression was established by both a H3K79 methylation-dependent and a methylation-independent mechanism; the latter required the histone acetyltransferase Gcn5. By monitoring the localization of a fluorescently tagged telomere in living cells, we found that the targeting of Dot1, but not its methylation activity, led to the release of a telomere from the repressive environment at the nuclear periphery. This probably contributes to the activity-independent derepression effect of Dot1. CONCLUSIONS: Targeting of Dot1 promoted gene expression by antagonizing gene repression through both histone methylation and chromatin relocalization. Our findings show that binding of Dot1 to chromatin can positively affect local gene expression by chromatin rearrangements over a considerable distance.

SUBMITTER: Stulemeijer IJ 

PROVIDER: S-EPMC3038881 | biostudies-other | 2011

REPOSITORIES: biostudies-other

altmetric image

Publications

Dot1 binding induces chromatin rearrangements by histone methylation-dependent and -independent mechanisms.

Stulemeijer Iris Je IJ   Pike Brietta L BL   Faber Alex W AW   Verzijlbergen Kitty F KF   van Welsem Tibor T   Frederiks Floor F   Lenstra Tineke L TL   Holstege Frank Cp FC   Gasser Susan M SM   van Leeuwen Fred F  

Epigenetics & chromatin 20110203 1


<h4>Background</h4>Methylation of histone H3 lysine 79 (H3K79) by Dot1 is highly conserved among species and has been associated with both gene repression and activation. To eliminate indirect effects and examine the direct consequences of Dot1 binding and H3K79 methylation, we investigated the effects of targeting Dot1 to different positions in the yeast genome.<h4>Results</h4>Targeting Dot1 did not activate transcription at a euchromatic locus. However, chromatin-bound Dot1 derepressed heteroc  ...[more]

Similar Datasets

| S-EPMC2680671 | biostudies-literature
| S-EPMC2064831 | biostudies-literature
| S-EPMC9379585 | biostudies-literature
| S-EPMC5940172 | biostudies-literature
| S-EPMC1994663 | biostudies-literature
| S-EPMC4628070 | biostudies-literature
| S-EPMC3730430 | biostudies-literature
| S-EPMC2888416 | biostudies-literature
| S-EPMC6182156 | biostudies-literature
| S-EPMC6348102 | biostudies-literature