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Improving sensitivity in nonlinear Raman microspectroscopy imaging and sensing.


ABSTRACT: Nonlinear Raman microspectroscopy based on a broadband coherent anti-Stokes Raman scattering is an emerging technique for noninvasive, chemically specific, microscopic analysis of tissues and large population of cells and particles. The sensitivity of this imaging is a critical aspect of a number of the proposed biomedical application. It is shown that the incident laser power is the major parameter controlling this sensitivity. By careful optimizing the laser system, the high-quality vibrational spectra acquisition at the multi-kHz rate becomes feasible.

SUBMITTER: Arora R 

PROVIDER: S-EPMC3061330 | biostudies-other | 2011 Feb

REPOSITORIES: biostudies-other

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