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Cutting edge: Hierarchy of maturity of murine memory B cell subsets.

ABSTRACT: The paucity of murine memory B cell markers has been a significant impediment to the study of memory. The most commonly used marker is IgG, which is neither sensitive nor specific, because activated nonmemory cells can be IgG(+), and memory cells can be IgM(+). In this article, we show that, together, PD-L2 (CD273), CD80, and CD73 define at least five phenotypic subsets of murine memory B cells. These subsets are generated from naive cells bearing a single BCR in response to a single T-dependent Ag. This diversity is independent of class switch, because IgG(1)- and IgM-bearing memory cells are found within each compartment. Memory subsets defined by PD-L2, CD80, and CD73 are biologically distinct from one another, because they differ in ontogeny and selection. Together, these distinctions suggest that there is a spectrum of memory B cells and progressive acquisition from more naive-like to more memory-like properties.

SUBMITTER: Tomayko MM

PROVIDER: S-EPMC3133669 | biostudies-other | 2010 Dec

REPOSITORIES: biostudies-other

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Cutting edge: Hierarchy of maturity of murine memory B cell subsets.

Tomayko Mary M MM Steinel Natalie C NC Anderson Shannon M SM Shlomchik Mark J MJ

Journal of immunology (Baltimore, Md. : 1950) 20101115 12

The paucity of murine memory B cell markers has been a significant impediment to the study of memory. The most commonly used marker is IgG, which is neither sensitive nor specific, because activated nonmemory cells can be IgG(+), and memory cells can be IgM(+). In this article, we show that, together, PD-L2 (CD273), CD80, and CD73 define at least five phenotypic subsets of murine memory B cells. These subsets are generated from naive cells bearing a single BCR in response to a single T-dependent ...[more]

PMID: 21078902

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