Ontology highlight
ABSTRACT:
SUBMITTER: Wang B
PROVIDER: S-EPMC3207208 | biostudies-other | 2011 Oct
REPOSITORIES: biostudies-other
Wang Baojun B Kitney Richard I RI Joly Nicolas N Buck Martin M
Nature communications 20111018
Modular and orthogonal genetic logic gates are essential for building robust biologically based digital devices to customize cell signalling in synthetic biology. Here we constructed an orthogonal AND gate in Escherichia coli using a novel hetero-regulation module from Pseudomonas syringae. The device comprises two co-activating genes hrpR and hrpS controlled by separate promoter inputs, and a σ(54)-dependent hrpL promoter driving the output. The hrpL promoter is activated only when both genes a ...[more]